my virus has a concentration of 7.7x10^9pfu/ml. i want to infect a 60mm dish with 100pfu.. how do i dilute

To determine how to dilute the viral stock, follow these steps:

1. **Stock concentration**: You have \( 7.7 \times 10^9 \) plaque-forming units (PFU) per milliliter (ml) of your virus.
2. **Desired amount**: You want to infect a 60 mm dish with 100 PFU.

The formula to use is:

\[
V = \frac{\text{Desired PFU}}{\text{Stock concentration}}
\]

Where:
- \(V\) is the volume of stock you need.
- Desired PFU = 100 PFU
- Stock concentration = \(7.7 \times 10^9 \, \text{PFU/ml}\)

Let's calculate this.

\[
V = \frac{100}{7.7 \times 10^9} = 1.3 \times 10^{-8} \, \text{ml}
\]

So, you'd need \(1.3 \times 10^{-8}\) ml of the stock solution, which is an impractically small volume. Thus, a dilution is necessary.

### Step 1: Choose a dilution factor
Let's say you choose a **dilution factor** of \( 1:10^8 \).

### Step 2: Dilute the stock solution
- First, dilute the virus solution by taking 1 ml of the stock and adding it to 99 ml of the diluent (to achieve \(10^2\)).
- Then, perform the next step: dilute 1 ml of the result into another 99 ml for a \(10^6\) dilution.
- Finally, dilute 1 ml from this result into 99 ml for a \(10^8\) dilution.

### Step 3: Use the diluted solution
From this final \(10^8\) dilution, you can use 1 ml to get your desired 100 PFU.

Would you like more details or clarification on any part of this process?

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Here are some related questions:
1. How does plaque-forming unit (PFU) relate to viral infectivity?
2. What are some common dilution techniques for viral assays?
3. How do you ensure accuracy when preparing large dilutions?
4. Why is it important to perform serial dilutions rather than single-step dilutions?
5. What impact does dish size have on viral concentration?

**Tip**: Always mix your dilutions thoroughly to ensure an even distribution of the virus.

Learn how to calculate the necessary dilution for a virus stock with a concentration of 7.7x10^9 PFU/ml to infect a 60mm dish with 100 PFU. This step-by-step guide covers key dilution concepts and provides the necessary formulas to achieve the desired viral concentration.

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